Average final volumes of oxygen for each concentration of hydrogen peroxide. Evaluation Overall, I believe my experiment went well and that I gained sufficient results because I repeated each concentration three times and investigated eight concentrations in total. I believe that my results were also relatively reliable because as the concentration decreased the volume of oxygen produced also decreased.
Investigating an enzyme-controlled reaction: Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell.
Cells make the enzyme catalase to remove hydrogen peroxide. This investigation looks at the rate of oxygen production by the catalase in pureed potato as the concentration of hydrogen peroxide varies. The oxygen produced in 30 seconds is collected over water.
Then the rate of reaction is calculated.
Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide. Individual students may then have time to gather repeat data.
Groups of three could work to collect results for 5 different concentrations and rotate the roles of apparatus manipulator, result reader and Catalase and hydrogen peroxide experiment coursework. Collating and comparing class results allows students to look for anomalous and inconsistent data.
Apparatus and Chemicals For each group of students: Wash splashes of pureed potato or peroxide off the skin immediately. Be aware of pressure building up if reaction vessels become blocked. Take care inserting the bung in the conical flask — it needs to be a tight fit, so push and twist the bung in with care.
Take care when removing the cap of the reagent bottle, as gas pressure may have built up inside. Dilute immediately before use and put in a clean brown bottle, because dilution also dilutes the decomposition inhibitor.
Keep in brown bottles because hydrogen peroxide degrades faster in the light. Discard all unused solution. Do not return solution to stock bottles, because contaminants may cause decomposition and the stock bottle may explode after a time. You might need to add water to make it less viscous and easier to use.
Discs of potato react too slowly. Wear eye protection and protect clothing from hydrogen peroxide.
Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals).It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Likewise, catalase has one of . Published: Wed, 23 May The investigation was carried out to determine how different ph levels and hydrogen concentrations, affect the activity of catalase in potato during the decomposition of hydrogen peroxide to produce water and oxygen. Experiment 6A Biology with Calculators 6A - 1 Enzyme Action: Testing Catalase Activity Many organisms can decompose hydrogen peroxide (H 2O 2) enzymatically. Enzymes are globular proteins, responsible for most of the .
Rinse splashes of peroxide and pureed potato off the skin as quickly as possible. Preparation a Make just enough diluted hydrogen peroxide just before the lesson. Set out in brown bottles Note 1. Investigation d Use the large syringe to measure 20 cm3 pureed potato into the conical flask.
Invert it over the trough of water, with the open end under the surface of the water in the bowl, and with the end of the rubber tubing in the measuring cylinder. Put the syringe in place in the bung of the flask, but do not push the plunger straight away.
Push the plunger on the syringe and immediately start the stopclock. Measure another 20 cm3 pureed potato into it.
Reassemble the apparatus, refill the measuring cylinder, and repeat from g to j with another concentration of hydrogen peroxide. Use a cm3 measuring cylinder for concentrations of hydrogen peroxide over 20 vol.
Teaching notes Note the units for measuring the concentration of hydrogen peroxide — these are not SI units. In this procedure, 2 cm3 of 10 vol hydrogen peroxide will release 20 cm3 of oxygen if the reaction goes to completion.
So if the apparatus is free of leaks, 22 cm3 of water should be displaced in the measuring cylinder with 10 vol hydrogen peroxide. Oxygen is soluble in water, but dissolves only slowly in water at normal room temperatures. Use this information as a check on the practical set-up. Values below 22 cm3 show that oxygen has escaped, or the hydrogen peroxide has not fully reacted, or the hydrogen peroxide concentration is not as expected.
Ask students to explain how values over 22 cm3 could happen. Liver also contains catalase, but handling offal is more controversial with students and introduces a greater hygiene risk.
Also, the reaction is so vigorous that bubbles of mixture can carry pieces of liver into the delivery tube. If collecting the gas over water is complicated, and you have access to a cm3 gas syringe, you could collect the gas in that instead. Be sure to clamp the gas syringe securely but carefully.
The reaction is exothermic.Apr 30, · Catalase is an enzyme which is found in most living organisms.
It catalyses the decomposition of hydrogen peroxide into water and oxygen. 2H 2 O 2 + Catalase >>> 2H 2 O + O 2.
Catalase dramatically reduces the activation energy needed for the caninariojana.coms: 5. - The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase.
When the enzyme catalase comes into contact with its substrate, hydrogen peroxide, it starts breaking it down into water and oxygen. Oxygen is a gas and therefore wants to escape the liquid. Catalase and hydrogen peroxide experiment coursework, - Publication editing.
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This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase.
Introduction This is an A-level biology project. - The Effect Of Catalase On Hydrogen Peroxide Planning Catalase is an enzyme found in the blood and in most living cells its job is to speed up the decomposition of hydrogen peroxide into water and oxygen.
2H2O2 + Catalase ¾¾¾¾¾¾¾® 2H2O + O2 The chemical compound hydrogen peroxide (H2O2) is a viscous liquid that has strong oxidizing.